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This allows experts to ascertain the light depth of the material and decide its concentration. The upper the extent of absorption at a particular wavelength, the upper the concentration of a compound.Identification of Substances: UV spectroscopy may also help establish the chemical identification of a compound. Distinctive compounds absorb UV light
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Analyte molecules partition amongst a liquid stationary stage along with the eluent. Just as in hydrophilic interaction chromatography (HILIC; a sub-strategy in just HPLC), this process separates analytes determined by variations of their polarity. HILIC most often uses a bonded polar stationary section as well as a mobile stage built mostly of ace
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